On day 0, 3.2 mg anti-collagen-II antibody mix was injected i.p. in DBA/J1 mice to induce collagen antibody induced arthritis (CAIA), followed by an i.p. injection containing 25 g LPS on day 3 together with second generation tACPA (1 mg/mouse) or without antibody (no Ab). Shown are the means SEM (n=5). Mice were scored daily for signs of inflammation in their paws. Scoring was performed according to the method outlined in Table 1.
Figure 9: Second generation tACPAs identified by phage display.