| Peptide Number |
Amino-acid sequence |
| 1 |
S G X G K Q G G K A R A |
| 2 |
A K A K S X S S R A G L |
| 3 |
K S R S S X A G L Q F P |
| 4 |
Q F P V G X V H R L L R |
| 5 |
V G R V H X L L R K G N |
| 6 |
V H R L L X K G N Y S E |
| 7 |
G N Y S E X V G A G A P |
| 8 |
A G N A A X D N K K T R |
| 9 |
D N K K T X I I P R H L |
| 10 |
T R I I P X H L Q L A I |
| 11 |
L Q L A I X N D E E L N |
| 12 |
N K L L G X V T I A Q G |
Synthesized peptides of all domains in histone-2A in which an arginine could be
enzymatically changed into a citrulline. Peptides were designed in such a way,
that the citrulline residue (X) was positioned approximately in the middle of the
peptide, with 5 and 6 amino acids to its left and right, respectively. Since the first
possible citrulline in histone-2A is positioned at the +3 position from the N-terminus
of the protein, three neutrally charged alanine residues were added, to position this
citrulline in a way comparable to its peers. |
