Figure 1: Immunodepletion of exosomes in viral preparations. NL4- 3Balenv virions were produced by transfection of HEK-293T cells. Culture supernatant was centrifuged and the pellet was re-suspended and incubated with protein A/G beads pre-coated with anti-AChE (AE-1) or with isotype control (IgG1) antibody. AChE activities associated with the A/G beads (immunocaptured exosomes), in the bead supernatant and in the original resuspended centrifugal pellet were measured