Figure 2: Release of exosomes from dendritic cells after HIV-1 capture. DCs were incubated for 1h with mock viral suspension or with HIV-1 clone JR-CSF and then cultured for an additional 24h, 48h or 96h. Exosomes and HIV-1 virions were separated by differential centrifugation of the cell-free supernatants and the amount of exosomes in the pellet was determined by means of an ELISA targeting the exosome marker HLA-DR. Data represent the mean SEM of triplicate samples for one donor and are representative of three independent experiments. Sequential Bonferroni correction indicated that p < 0.01.