Figure 5: Effect of released particles on CD4TL death. Resting CD4TL (panel a) or PHAL/IL-2-activated CD4TL (panel b) were incubated for 5d with exosomes and attenuated HIV-1 obtained from the 100,000xg pellet from culture of NL4-3Balenv-infected DCs. The fluorescent pan-caspase inhibitor (FITC-VAD-FMK) was added 30 min before flow cytometry analysis. Results are expressed as the relative increase compared to the control condition without exosome. Data represent the mean ± SEM from five independent experiments and the asterisk denotes statistically significant data (P < 0.05).