Figure 5: Effect of released particles on CD4TL death. Resting CD4TL (panel a) or PHAL/IL-2-activated CD4TL (panel b) were incubated for 5d with exosomes and attenuated HIV-1 obtained from the 100,000xg pellet from culture of NL4-3Balenv-infected DCs. The fluorescent pan-caspase inhibitor (FITC-VAD-FMK) was added 30 min before flow cytometry analysis. Results are expressed as the relative increase compared to the control condition without exosome. Data represent the mean SEM from five independent experiments and the asterisk denotes statistically significant data (P < 0.05).