Figure 4: IL-7 induced pSTAT-5 of co-cultured thymocytes. Thymocytes were co-cultured with TEC or OP9-DL1 cells for 96 hours and then stimulated with IL-7 (1 ng/ml) for 15 minutes. The level of STAT-5 phosphorylation was measured by intracellular staining. Thymocytes within OP9-DL1 cocultures had a higher level of basal STAT-5 phosphorylation than thymocytes with TECs. n=3 p=0.007 by student t-test.