Figure 5: IL-7 enhances thymocytes survival of co-cultured thymocytes. A) Thymocytes were cocultured with TEC or OP9-DL1 cells for 96 hours and then were stimulated with IL-7 (1 ng/ml) for 48 hours. Bcl-2 expression was measured by intracellular staining. Thymocytes co-cultured with OP9- DL1 had a significantly higher level of Bcl-2 expression in response to IL-7 than thymocytes cocultured with TEC. n=4 p=0.003 by student t-test. B) Thymocytes were co-cultured with TEC or OP9-DL1 cells and stimulated with IL-7 for 96 hours. Glucose uptake was measured in the thymocytes by 3H-Dglucose. Thymocytes co-cultured with OP9-DL1 had a significantly higher level of glucose uptake in response to IL-7 than thymocytes co-cultured with TEC. n=3 p=0.007 by student t-test.