Figure 2: Oral and topical administration of Compound A attenuated skin inflammation after oxazolone challenge in C57BL/6 mice.
Changes in the mouse ear thickness after oral dosing a), or topical application b) of either Compound A, vehicle or Clobetasol after once a-b) or twice c) oxazolone challenges. d) Representative H&E sections from mouse ears collected at termination of the study described in Figure 2b). e) RNA was isolated from mouse ears at study termination described in Figure 2b). Quantitative RTPCR for various cytokine transcripts was performed using prequalified primers and probes on ear RNA. Results reported as group means SEM, results are representative of at least 3 studies, for experiments shown 8-10 mice were used per treatment group.