Fertilisation volume (mL)

Mean normal larval hatch-out rate (%)

Mean abnormal larval hatch-out rate (%)

Mean unfertilised eggs rate (%)

1

20±4.0a

9.0±5.0

71±7.0

4

75±7.0b

14±6.0

12±6.0

10

95±1.0b

3.0±1.0

2.0±0.0

50

76±10b

1.0±0.0

22±11

100

93±3.0b

1.0±0.0

5.0±4.0

200

97±0.0b

2.0±0.0

1.0±0.0

a, b Similar superscripts indicate no significant difference (P > 0.05)
Table 2: Fertilisation and hatch-out success rates (mean ± SEM) of H. midaelarvae relative to fertilisation volume.