Treatment
(three replicates of 10 crickets)

Exposure to GFP

Day of experiment of exposure

Unhandled controls

N/A

N/A

F1

Food

Day 1 only

F2

Food

Day 1, 7 and 14

F3

Food

Daily

W1

Water

Day 1 only

W2

Water

Day 1, 7 and 14

W3

Water

Daily

Table 1: Experimental design for exposure of the house cricket (Acheta domesticus) to gfp expressed plasmids in Escherichia coli.