Treatment

Exoskeleton

Muscle

Proventriculus

Proventriculus associated tissues

Unhandled controls

0% (+++)

0% (+++)

0% (+++)

0% (+++)

 

0% (++)

0% (++)

0% (++)

0% (++)

 

0% (+)

0% (+)

0% (+)

0% (+)

Total

0%

0%

0%

0%

Food-Day 1

0% (+++)

0% (+++)

0% (+++)

0% (+++)

 

0% (++)

20% (++)

40% (++)

20% (++)

 

0% (+)

0% (+)

20% (+)

0% (+)

Total

0%

20%

60%

20%

Food-Day 1, 7, 14

0% (+++)

50% (+++)

50% (+++)

66.6% (+++)

 

0% (++)

0% (++)

12.5% (++)

33.3% (++)

 

0% (+)

25% (+)

25% (+)

0% (+)

Total

0%

75%

87.5%

99.9%

Food-Daily

33% (+++)

28% (+++)

53.8% (+++)

38.4% (+++)

 

0% (++)

7% (++)

23% (++)

38.4% (++)

 

0% (+)

43% (+)

15% (+)

15% (+)

Total

33%

78%

91.8%

91.8%

Water-Day 1

10% (+++)

10% (+++)

12.5% (+++)

28% (+++)

 

0% (++)

0% (++)

50% (++)

28% (++)

 

0% (+)

40% (+)

12.5% (+)

28% (++)

Total

10%

50%

75%

84%

Water-Day 1, 7, 14

0% (+++)

0% (+++)

28% (+++)

0% (+++)

 

0% (++)

0% (++)

0% (++)

14% (++)

 

0% (+++)

0% (+)

28% (+)

0% (+)

Total

0%

0%

56%

14%

Water-Daily

0% (+++)

0% (+++)

42% (+++)

20% (+++)

 

0% (++)

0% (++)

28% (++)

0% (++)

 

0% (+)

0% (+)

14% (+)

40% (+)

Total

0%

0%

84%

60%

+++ Bright fluorescence
++ Medium fluorescence
+ Low fluorescence
*Percentage is based on the number of crickets for which the tissues could be
analysed. In some circumstances, one or two of the above tissues may have been
absent from the cricket sections due to the processing and angle of cutting of the
longitudinal section of the cricket.
Table 2: Summary of Acheta domesticus tissues exhibiting fluorescence following exposure to bacterially expressed gfp via food or water (grading of fluorescence is represented in Figure 1)*.