Primers Sequence Tm GC% MtAro268F 5'-GCCAGYAACTACTACAACRACAAG-3' 62-65 42-50 MtAro698F 5'-TTTACACRTGGCAGACTGTR-3' 57-61 43-52 MtAro1384R 5'-GGATGGAYTTCATCATCACCATG-3' 61-63 43-48 MtAro1476R 5'-GGCTGCTGGASAGGTTGTTG-3' 61-62 43-44

Table 1: List of degenerate primers designed for tomcod CYP19A partial sequence.