Figure 1: Agarose gel electrophoresis images of the PCR products obtained by 19 primer pairs using a 55°C annealing temperature. A-S correspond to the primer pairs shown in Table 1. The left-most lane (1st lane) of each gel image is the size marker (1 Kb Plus DNA Ladder, Invitrogen), and the 2nd-6th lanes correspond to long-spined sea urchin (Diadema savignyi), neon flying squid (Ommastrephes bartramii), golden crab (Chaceon granulatus), mako shark (Isurus oxyrinchus), and skipjack tuna (Katsuwonus pelamis), respectively.