Pair Gene Primer Orientation Primer sequence (5'-3') Distance* A S2 RPS2exCF forward CCHGTNCCCAAGAAGCTGCT 70   S2 RPS2exDR reverse GGTYTCCTTCCASAGRTCAG 43 B S3 RPS3ex1F forward AAATCTCVAAGAAGAGRAAG 2   S3 RPS3ex2R reverse ACRCCGGAGTANCCATCCTC 62 C S3 RPS3ex2F forward GAGGATGGNTACTCCGGYGT 53   S3 RPS3ex3R reverse CKCTTCTGVACMACAGCGGT 48 D S3 RPS3ex3F forward ACCGCTGTKGTBCAGAAGMG 30   S3 RPS3ex4R reverse GTCGCAACCTTCTCAGCRTA 24 E S3 RPS3ex4F forward TAYGCTGAGAAGGTTGCGAC 74   S3 RPS3ex5R reverse AASCKCAGRACACCATARCA 6 F S14 RPS14exAF forward GCCTCMTTYAACGAYACCTT 29   S14 RPS14exBR reverse CKGTCRGCCTTYACCTTCAT 30 G S17 RPS17exBF forward AGWGGMATCTCCATCAARCT 49   S17 RPS17exCR reverse GGRTCRACCTCGATGATSTC 18 H S23 RPS23exAF forward TCACACGCCAARGGCATYGT 15   S23 RPS23exBR reverse ATRGCAGAGTTRGGCTGCTT 14 I S23 RPS23exBF forward AAGAACGGCAARAAGATCAC 42   S23 RPS23exCR reverse TGACCYTTACGWCCRAATCC 26 J S27 RPS27exAF forward GCTAARATYCARGAYAAGGA 4   S27 RPS27exBR reverse GTGCGKCCRTCYTCCAGCTG 43 K L3 RPL3exAF forward GGCTACAAGGCYGGYATGAC 39   L3 RPL3exBR reverse CAGTTYTTGTAGAAKCGACG 149 L L3 RPL3exBF forward GCGTCGMTTCTACAARAACT 2   L3 RPL3exCR reverse GTGAARGCCTTCTTCTYGGA 9 M L3 RPL3exDF forward CAAGGGWCACGGRTDCAAGG 2   L3 RPL3exER reverse GTCTTRCGGGGMAGCTTCTT 25 N L8 RPL8exBF forward AGTTCATCTACTGCGGCAAG 37   L8 RPL8exCR reverse ATGAYGGTDCCYTCAGGCAT 5 O L9 RPL9exCF forward RAACTTCYTGGGRGAGAAGT 110   L9 RPL9exDR reverse ACDGTGGTGGCYTGCTGGAT 10 P L11 RPL11exAF forward CARACGCCHGTCTTCTCCAA 5   L11 RPL11exBR reverse AAGGATCKYACAGTGTAGCG 4 Q L11 RPL11exBF forward CGCTACACTGTRMGATCCTT 87   L11 RPL11exCR reverse CCCARRTCGATGTGYTCCTG 65 R L17 RPL17exAF forward GAGWCMGCTCAGGCCATCAA 132   L17 RPL17exBR reverse TCGGCRCTCTTCTTRGGCCA 8 S L24 RPL24exBF forward AGMCGCAARCACAAGAAGGG 13   L24 RPL24exCR reverse ACYTCAGGCTTCTGRTTCCT 85 *Number of nucleotides between 3’-end of the primers and start or end of the target introns

Table 1: Sequences of primers for amplifying 19 introns of 12 ribosomal protein genes.