Histological
Figure 2: Histological analysis of P. incognitusgills (A,B) and liver (C-F) from reference site (A,C,E) and polluted Sousse bay site (B,D,E) colored by Masson trichrome. (A) Reference gills section showing the filament (GF), primary lamellae (PL), secondary lamellae (SL), pillar cell (PC), chondrocytes (Ch), gill epithelium (GE), chloride cells (CC) and erythrocytes (Er) (X40). (B) Gills section from fish of the contaminated sites howing a single stratum epithelium (SSE), fusion of secondary lamellae (FSL), telangiectasis (T), epithelium lifting (EL), shorting of secondary lamellae (SSL) and disappearance of chondrocytes DC (X40). (C) Liver section in reference P. incognitus showing sinusoids (S) and central vein (CV) (X40). (D) Liver section in P. incognitus from the contaminated site showing hypertrophy of hepatocytes (HH) congested and dilated sinusoids (C/DS) and congested and dilated central vein (C/DCV) (X40). (E) Liver section in reference P. incognitus showing hepatocytes (H) with basophilic. granulation (BG) and central and round nucleus (N) (X100). (F) Liver section in P. incognitus from the contaminated site showing pyknotic nucleus (PN) and hepatic steatosis (HS) (X40)..