Biomarker |
Reference Site |
Contaminated site |
% |
Gills |
|
|
|
Superoxidedismutase (SOD) |
41.925 ± 8.985a,b |
32.526 ± 11.780a,b |
76%f |
Catalase (CAT) |
3.106 ± 1.058a,c |
1.390 ± 0.512a,c* |
45% |
Glutathioneperoxidase (GPx) |
1195.311 ± 520.825a,d |
1154.000 ± 173.30a,d |
N.A. |
Lipidperoxidation (MDA) |
0.040 ± 0.005a,e |
0.0510 ± 0.005a,e** |
127.50% |
IntegratedBiomarkerResponse (IBR) |
0.812 |
1.364 |
|
Liver |
|
|
|
Superoxidedismutase (SOD) |
36.923 ± 11.845a,b |
26.937 ± 10.495a,b* |
72% |
Catalase (CAT) |
15.294 ± 11.888a,c |
79.745 ± 40.780a,c |
N.A. |
Glutathioneperoxidase (GPx) |
973.373 ± 405.460a,d |
470.466 ± 282.908a,d* |
48% |
Lipidperoxidation (MDA) |
0.037 ± 0.007a,e |
0.063 ± 0.007a,e** |
170% |
IntegratedBiomarkerResponse (IBR) |
0 |
7.025 |
|
|
aData are represented as the means ± SD; n = 10. Statistical differences between contaminated and reference groups were made at 0.05 confidence level. Statistical
significance of the results is indicated as *, p<0.05; **, p<0.01. Enzymatic activity units are bμmol/min/mg protein for SOD, cμmol H2O2 consumed/min/mg protein for
CAT, dμmol NADPH/min/mg protein for GPx, and e nmol TBARS/mg protein for MDA level.f For the oxidative stress biomarkers, the value measured in samples from the
contaminated site is given as the % of the reference value when a significant difference was observed. N.A., not applicable. |