Biomarker Reference Site Contaminated site %
Gills      
Superoxidedismutase (SOD) 41.925 ± 8.985a,b 32.526 ± 11.780a,b 76%f
Catalase (CAT) 3.106 ± 1.058a,c 1.390 ± 0.512a,c* 45%
Glutathioneperoxidase (GPx) 1195.311 ± 520.825a,d 1154.000 ± 173.30a,d N.A.
Lipidperoxidation (MDA) 0.040 ± 0.005a,e 0.0510 ± 0.005a,e** 127.50%
IntegratedBiomarkerResponse (IBR) 0.812 1.364  
Liver      
Superoxidedismutase (SOD) 36.923 ± 11.845a,b 26.937 ± 10.495a,b* 72%
Catalase (CAT) 15.294 ± 11.888a,c 79.745 ± 40.780a,c N.A.
Glutathioneperoxidase (GPx) 973.373 ± 405.460a,d 470.466 ± 282.908a,d* 48%
Lipidperoxidation (MDA) 0.037 ± 0.007a,e 0.063 ± 0.007a,e** 170%
IntegratedBiomarkerResponse (IBR) 0 7.025  
aData are represented as the means ± SD; n = 10. Statistical differences between contaminated and reference groups were made at 0.05 confidence level. Statistical significance of the results is indicated as *, p<0.05; **, p<0.01. Enzymatic activity units are bμmol/min/mg protein for SOD, cμmol H2O2 consumed/min/mg protein for CAT, dμmol NADPH/min/mg protein for GPx, and e nmol TBARS/mg protein for MDA level.f For the oxidative stress biomarkers, the value measured in samples from the contaminated site is given as the % of the reference value when a significant difference was observed. N.A., not applicable.
Table 2: Oxidative stress biomarkers and Integrated Biomarker Response (IBR) in gills and liver of P.incognitusfishes from reference and contaminated sites.