Figure 1: Cardiovascular defects induced in Tg(fil-1:EGFP) zebrafish at 2 dpf following exposure to 1 μM of 4-t-OP. Lateral view of (A, C and E) control Tg(fil-1:EGFP) zebrafish and (B, D and F) 1 μM 4-t-OP treated Tg(fil-1:EGFP) embryos. (A, B, C and D) Photos were acquired by merging bright field and dark field using FITC filter. (E and F) Photos were acquired form dark field using FITC filter.