Figure 3: Quantitative PCR analyses of gene expression in 4-t-OP exposed Tg(fil-1:EGFP) zebrafish embryos at 48 and 72 hpf. The expression of ERα, ERβ1 and ERβ2 were quantified. The data of the control group at 48 hpf was set as 1. The results represented triplicated experiments, and the different letters were considered significantly at p<0.05.