| Gene name |
Primer sequence |
PCR size (bp) |
Accession number |
| (5’→3’) |
| Estrogen receptor a (ERa) |
F:CCGGCCCTACACAGAGATCA |
150 bp |
NM_152959 |
| R:AGCCAAGAGCTCTCCAACAACT |
| Estrogen receptor b1 (ERb1) |
F: CTGTGCCGTCTGCAGTGATT |
150 bp |
AF516874 |
| R: CGGCGGTTCTTGTCGATAGT |
| Estrogen receptor b2 (ERb2) |
F: TCCGACACCTCAGCAACAAA |
150 bp |
AF349413 |
| R: TTTCTGGGCTCTGTTGTCTGTCT |
| NK2 homeobox 5 (Nkx2.5) |
F: CGGGATGGTAAACCGTGTCT |
150 bp |
NM_131421 |
| R: GCTCGACGGATAGTTGCATGA |
| NK2 homeobox 7 (Nkx2.7) |
F: AGCTCACATCCACACAGGTCAA |
150 bp |
NM_131419 |
| R: GAGCTCCGTGACAGGGTTTG |
| Heart and neural crest derivatives expressed 2 (Hand2) |
F: TGTCATGAAGAACCCCCCTAT |
150 bp |
NM_131626 |
| R: CCCCGGTACTCCTCCGTAGT |
| GATA-binding protein 4 |
F: CCAGTCTGCAACGCATGTG |
150 bp |
NM_131236 |
| (GATA-4) |
R: GATCGCCGACTGACCTTCAG |
| GATA-binding protein 5 |
F: GGGACGCCAGGGAACTCTA |
150 bp |
NM_131235 |
| (GATA-5) |
R: CACGCGTTGCACAGGTAGTG |
| GATA-binding protein 6 |
F:AGTCGCGACCAGTACCTTTCAA |
150 bp |
NM_131557 |
| (GATA-6) |
R: CCTTCGGGATTGCAGTGAGT |
| Fibroblast growth factor 1a |
F: ATGGCAAGCTGTACGCTTCA |
150 bp |
NM_200760 |
| (FGF1a) |
R: GGCCCCGTTTCATTTTCC |
| T-box 2a (Tbx2a) |
F:ACGTTTTCCCTGAGACCGATT |
150 bp |
AF179405 |
| R:ATGGAAGGGTCAGCTGTTTCC |
| T-box 2b (Tbx2b) |
F: ACGTTTTCCCTGAGACCGATT |
150 bp |
NM_131051 |
| R:ATGGAAGGGTCAGCTGTTTCC |
| T-box 5a (Tbx5a) |
F: CGGATGTTTCCCAGCTTCAA |
150 bp |
NM_130915 |
| R: CATCGCAGGCTCAGCTTTC |
| Elongation factor 1a (ef-1a) |
F: TGGTGGTGTCGGTGAGTTTG |
150 bp |
AY422992 |
| R: AAACGAGCCTGGCTGTAAGG |
