4t-OP conc. (mM) Survival rate (%)a Hatching rate (%)a Malformation rate (%)a
Control (0 mM)b 96 ± 2.1% 96 ± 2.9% 0%
0.1 mM 95 ± 1.8% 95 ± 1.1% 0%
0.5 mM 79 ± 1.5% 83 ± 1.7% 28 ± 1.6%
1.0 mM 52 ± 1.8% 71 ± 2.8% 60 ± 1.3%
2.5 mM 26 ± 2.8% 45 ± 2.3% 87 ± 4.4%
5.0 mM 12 ± 0.4% 23 ± 1.8% 100%
aData was evaluated from three times experiment and each experiment was triplicated (n=100).
bControl indicated the embryo medium only containing 0.2% PTU.
Table 2: Survival rate, hatching rate and malformation rate of zebrafish embryos exposed to different concentration of 4-t-OP at 3 dpf.