Developmental stages Treatment Number of embryos examined Embryos with cardiovascular defects
Pericardial edema Irregular shape of atrium and ventricle Abnormal blood vessel development
 
1 dpf Control 312 None None None
1mM 4t-OP 287 123 (43%) 49 (17%) 26 (9%)
2 dpf Control 304 None None None
1mM 4t-OP 273 153 (56%) 76 (28%) 44 (16%)
3 dpf Control 301 None None None
1 mM 4t-OP 178 110 (62%) 83 (47%) 66 (37%)
4 dpf Control 297 None None None
1 mM 4t-OP 143 61 (43%) 31 (21%) 67 (47%)
Zebrafish embryos were exposed to control (embryo medium contain PTU only) or 1 μM 4t-OP and the cardiovascular defects was examined on days 1, 2, 3 and 4. Data shown are the pooled results of triplicated experiments.
Table 3: Cardiovascular defects induced by 4t-OP.