| Developmental stages |
Treatment |
Number of embryos examined |
Embryos with cardiovascular defects |
| Pericardial edema |
Irregular shape of atrium and ventricle |
Abnormal blood vessel development |
| |
| 1 dpf |
Control |
312 |
None |
None |
None |
| 1mM 4t-OP |
287 |
123 (43%) |
49 (17%) |
26 (9%) |
| 2 dpf |
Control |
304 |
None |
None |
None |
| 1mM 4t-OP |
273 |
153 (56%) |
76 (28%) |
44 (16%) |
| 3 dpf |
Control |
301 |
None |
None |
None |
| 1 mM 4t-OP |
178 |
110 (62%) |
83 (47%) |
66 (37%) |
| 4 dpf |
Control |
297 |
None |
None |
None |
| 1 mM 4t-OP |
143 |
61 (43%) |
31 (21%) |
67 (47%) |
|
| Zebrafish embryos were exposed to control (embryo medium contain PTU only)
or 1 μM 4t-OP and the cardiovascular defects was examined on days 1, 2, 3 and
4. Data shown are the pooled results of triplicated experiments. |
| Table 3: Cardiovascular defects induced by 4t-OP. |
