Figure 2a: Specificity of the hly gene-based PCR. Lanes 1 and 7,100 bp ladder (Takara Bio Inc.) 2, Vibrio campbellii ATCC 25920T; 3, Vibrio harveyi and V. Parahaemolyticus; 6, E. Coli C600. PCR products (5 μl each) were analysed by 1.5% agarose gel electrophoresis and visualized after ethidium bromide staining using Gel-doc 2000 (Bio-Rad, CA, USA).