Primary Serum

Clone

Type

Source

Titer

Antigen Retrieval

Cyclin A

 

Monoclonal

NEOMARKERS

1:200

CITRATE pH 6.0

Cyclin B1

V152

Monoclonal

DAKO

1:1500

EDTA+TRIS pH 9.0

Cyclin D1

RBT-14

Monoclonal

BIO-SB

Pre-diluted

EDTA+TRIS PH 9.0

p16

F12

Policlonal

SANTA CRUZ

1:400

CITRATE pH 6.0

p21

SX118

Policlonal

DAKO

1:50

CITRATE pH 6.0

p27

SX53G8

Monoclonal

DAKO

1:200

CITRATE pH 6.0

p53

DO-7

Monoclonal

DAKO

1:2000

CITRATE pH 6.0

Topoisomerase 2A

SWT-3D1

Monoclonal

DAKO

1:800

CITRATE pH 6.0

KI-67

MIB-1

Monoclonal

DAKO

1:300

CITRATE pH 6.0

Rb

NCL-RB358

Monoclonal

NOVOCASTRA

1:300

CITRATE pH 6.0

Table 2: Primary serum, clones, source, titer and antigen retrieval.