Clinical stage Histological grade Lymph node status Vascular embolization Perineural infiltration Recurrence
    I – II III – IV Well differentiated Poor/moderated differentiated Negative Positive No Yes No Yes No Yes
Cyclin A N 30 84 88 24 48 55 58 44 55 46 75 39
  Mean 8.49 9.53 8.62 11.18 9.50 10 9.25 9.82 8.88 10 9.99 7.84
  SD 10.54 9.77 9.42 11.20 11.47 9.26 10.86 9.83 10.15 10.51 11.1 7.13
  P value 0.376   0.311   0.349   0.543   0.551   0.788  
Cyclin B1 N 34 99 103 27 56 65 69 50 64 54 87 46
  Mean 12.61 14.16 13.01 16.73 15.23 13.20 12.72 15.32 14.67 12.70 14.51 12.37
  SD 14.16 14.17 13.63 16.16 14.99 13.94 12.83 15.68 14.98 12.90 14.36 13.74
  P value 0.517   0.287   0.533   0.626   0.540   0.224  
Cyclin D1 N 34 99 104 26 56 65 68 51 64 54 87 46
  Mean 33.60 40.34 37.77 42.66 33.96 44.63 38.18 41.05 38.49 40.28 38.42 38.98
  SD 20.76 20.55 21.01 17.97 20.47 20.06 21.69 18.90 20.76 19.98 19.39 23.29
  P value 0.084   0.264   0.006   0.298   0.534   0.876  
Topo IIA N 34 99 103 27 57 64 68 51 64 54 87 46
  Mean 23.13 25.16 23.23 28.92 25.27 24.29 26.21 24.60 26.51 24.37 24.40 25.11
  SD 17.77 16.80 16.36 18.52 16.87 17.38 16.89 17.14 16.69 17.04 17.48 16.26
  P value 0.456   0.163   0.693   0.562   0.402   0.613  
Ki-67 N 33 99 102 27 56 65 69 50 64 54 86 46
  Mean 33.70 31.61 31.88 32.80 33.23 31.37 33.34 31.64 33.71 31.02 32.09 32.22
  SD 14.71 15.17 13.58 18.46 14.73 15.66 16.47 13.75 14.49 16.33 14.22 16.60
  P value 0.457   0.806   0.420   0.919   0.265   0.956  
P16 N 35 99 104 27 57 65 69 51 65 54 88 46
  Mean 41.67 42.19 41.43 41.49 43.96 40.20 41.20 43.03 43.33 40.98 44.16 38.02
  SD 23.77 23.10 23.55 21.44 25.24 22.27 23.58 23.84 24.40 22.40 23.01 23.24
  P value 0.925   0.918   0.382   0.667   0.575   0.185  
P21 N 28 87 90 23 47 58 57 45 57 45 75 40
  Mean 15.64 11.90 12.37 15.29 13.34 12.21 10.90 14.03 12.83 11.32 13.93 10.71
  SD 12.37 10.32 10.94 10.85 11.20 10.79 9.96 10.94 11.30 9.48 11.13 10.32
  P value 0.142   0.189   0.668   0.129   0.639   0.124  
P27 N 29 87 89 26 47 59 57 46 57 45 77 39
  Mean 31.48 28.34 28.41 31.91 30.04 28.03 31.13 26.41 30.25 28.26 31.12 25.19
  SD 17.70 19.22 18.70 19.67 18.00 19.46 17.03 20.24 18.75 18.10 19.35 17.31
  P value 0.321   0.389   0.474   0.108   0.652   0.124  
P53 N 30 80 90 26 48 60 58 47 58 46 77 41
  Mean 49.00 46.28 45.47 52.27 49.33 48.53 49.87 46.94 46.36 50.24 48.77 43.60
  SD 33.50 32.57 33.86 27.07 33.35 32.74 31.11 34.47 32.28 33.69 32.27 33.58
  P value 0.462   0.389   0.961   0.709   0.596   0.432  
Rb N 31 95 98 26 53 62 65 49 65 48 82 44
  Mean 42.52 52.42 52.81 44.47 49.37 52.54 53.26 50.58 52.92 50.48 49.60 52.80
  SD 25.94 24.35 24.17 25.87 25.26 24.28 25.64 23.80 24.39 25.27 23.91 26.61
  P value 0.269   0.127   0.596   0.593   0.697   0.471  
SD:standard deviation
Table 3: Correlation between the expression of cell cycle proteins and clinicopathological parameters in patients with OSCC by IHC.