Figure 2: A) The acetaldehyde emitted in the bioreactor headspace of neutrophils (red bar) and PBMCs (blue bar) following 24 h of incubation. Acetaldehyde concentration is significantly higher from neutrophils (p=0.014) and significantly lower from PBMCs compared to media (white bar, p=0.004). X represents replicates. (B) Representative chromatograms of acetaldeyde from neutrophils (in red), PBMCs (in blue), and culture media (black). Acetaldehyde has been generated from neutrophils, however, PBMCs from the same subject presented a level below the culture medium. (C) Representative chromatograms of acetaldeyde in the presence of an additional 100 mg/L alanine. Neutrophils cultured with additional alanine (in red) emitted 1.5 fold higher amounts of acetaldehyde when compared to the neutrophils from the same subject cultured in basal medium. PBMCs from the same subject presented a level below the culture medium even in the presence of alanine (in blue).