Figure 5: Representative chromatograms of media (dashed) and media exposed to plastic EDTA vacutainers. These gases, 3-methyl pentane, n-hexane, and methylcyclopentane were observed to be significantly higher from PBMCs (in blue line) and/or neutrophils (in red line) in culture. However, they were determined to be carry-over contaminants from the plastic EDTA vacutainers, and excluded from the study.