Figure 1: GAL knockdown by siRNA on OVCA 429 cells. Western blot analysis of siRNA transfected OVCA 429 cells for NGAL (top panel) and β-actin (bottom panel). Positive control siRNA against β-actin and negative control siRNA were included, as well as mock transfected cells (transfection reagent but no siRNA complex). NGAL expression was significantly lower in cells treated with NGAL siRNA (* P<0.05 vs. mock transfected cells).