Figure 3: Wounding Assay of siRNA-transfected OVCA 429 cells. NGAL siRNA-treated cells and mock transfected cells were grown in a 6-well plate and wounded, as described in Materials and Methods. Measurements were taken between wound edges at time of wound (0 h) and 24 h and 48 h after wound (72 and 96 h post transfection). The percentage wound closure was calculated for both time points compared to 0 h. For both NGAL siRNA and mock transfected cells, there was a significant increase in wound closure between 72 and 96 h (*P<0.05 vs. 96 h mock transfected). At 96 h there was a significant increase in wound closure for NGAL siRNA-treated cells (**P<0.05 vs. 96 h NGAL siRNA).