Marker

Tumor Location

Stain Intensity

RFS

OS

 

 

Median

HR* (95% CI+)

p

HR (95% CI)

p

HIF-1α

Central

1

0.73 (0.22-2.41)

0.60

0.67 (0.22-2.08)

0.49

 

Peripheral

1

6.65 (1.14-38.97)

0.04

8.65 (1.78-42.06)

<0.01

 

All Tumor

1

0.92 (0.29-2.89)

0.89

1.01 (0.35-2.96)

0.98

PDK-1

Central

1

0.63 (0.20-2.02)

0.44

0.77 (0.26-2.33)

0.65

 

Peripheral

1

4.03 (0.65-24.93)

0.13

3.24 (0.84-12.46)

0.09

 

All Tumor

1

1.89 (0.59-5.99)

0.28

1.62 (0.54-4.82)

0.38

mTOR

Central

0.5

0.20 (0.05-0.88)

0.03

0.23 (0.06-0.87)

0.03

 

Peripheral

0.67

1.38 (0.25-7.54)

0.71

0.70 (0.16-3.11)

0.63

 

All Tumor

0.5

0.10 (0.01-0.66)

0.01

0.12 (0.02-0.64)

0.01

pAMPK

Central

0

0.21 (0.05-0.86)

0.03

0.48 (0.15-1.56)

0.22

 

Peripheral

0.17

0.54 (0.15-1.93)

0.34

1.78 (0.62-5.09)

0.28

 

All Tumor

0.25

0.27 (0.08-0.90)

0.03

0.68 (0.24-1.95)

0.47

*Hazard ratio with 95% +Confidence Interval
Table 3: Multivariate impact of high (above median stain score) metabolic marker staining intensity on overall survival (OS) and recurrence free survival (RFS).