Figure 2: M2BP was immunoprecipitated from the culture supernatant and human sera. A) Western blotting analysis of culture supernatant was performed using anti-M2BP and 1116NS19-9 and KM231 antibodies. B) Immunoprecipitated M2BP proteins were digested with PNGase F to remove the N-glycan. Western blotting analysis was then performed on the digested samples using anti-M2BP and 1116NS19-9 antibody. Closed arrowhead indicates the N-glycosylated M2BP and open arrowhead indicates the non-(N-)glycosylated M2BP. This figure was prepared from the results of two images. C) Western blotting analyses were performed using anti-M2BP and 1116NS19-9 antibody with sera of normal healthy volunteers and pancreatic cancer patients. Arrowhead indicates molecular weight of M2BP. D) Western blotting analyses were performed using anti-M2BP, 1116NS19-9 and KM231 antibodies with sera of normal healthy volunteers and pancreatic cancer patients.