Figure 4: Sandwich ELISA assay using a combination of anti-M2BP and 116NS19-9 antibody. Medium was used for the blank sample. (A) Capan-2 and PANC1 were used as positive and negative control, respectively. Capan-2 supernatant was serial diluted to 1/2-1/512. The background level (negative control) OD450 was ~0.2. (B) Eight cultured supernatants of pancreatic cancer cell lines were validated with sandwich ELISA. The background level (negative control) OD450 was ~0.1.