Figure 1: Urinary proteins separation by one-dimensional SDS gel: Urinary protein (25 μg/lane) from single sample and pooled samples were applied on a 12% acrylamide gel. Gel was stained by colloidal Coomassie. M = molecular marker; HC= healthy controls from non-endemic regions; EHC = endemic healthy controls; D0 = active VL patients (day-0); Dis = Patients after 30 days treatment; 6M = cured VL after 6 month follow-up.