Figure 3: Effect of TOX3 over-expression or siRNA mediated knockdown.
A) “Interferon Signaling Pathway”. Transcripts upregulated in TOX3 over expressing T24 bladder cancer cells at 16 hours post-transfection are marked yellow (log2 ratio >1 or -1).
B) Microarray expression profiling data of mock treated T24 cells or T24 cells over expressing TOX3 (ExonST1.0 arrays, RMA normalized) showed a strong upregulation of TOX3 as well as STAT1 upon TOX3 overexpression.
C) RT-qPCR validation of TOX3 and STAT1 expression in mock treated T24 cells or T24 cells over expressing TOX3 confirmed the upregulation identified by microarray profiling on ExonST1.0 arrays.
D) siRNA mediated partial knockdown of TOX3 in humanRT4 bladder cancer cells with high endogenous expression applying 20nM or 100nM siRNA resulted in a decrease of the TOX3 transcript compared to the scrambled siRNA used as a control. This downregulation of the TOX3 transcript resulted in a decrease of the STAT1 transcript.