Figure 1: (A) The amplified BHUP1 gene (1959BP) from promastigotes parasite DNA by PCR. Lane 1: 1kb DNA ladder and Lane 2: amplified hsp70 gene. (B) Electrophoretic analysis of pTZ57R/BHUP1 constructs. Lane 1: 1kb DNA ladder, Lane 2: plasmid digested with BamH1/EcoR1 restriction enzymes and Lane 3: uncut plasmid. (C) Ligation of clone BHUP1 gene into expression vector pET28a (+). Lane 1: DNA Ladder, Lane 2: digested pET28a vector and Lane 3: Ligated pET28a vector.