Disease Sample (patients & control) Pattern Origin Method Biomarkers Validation
HNSCC[41] 8&8 USS WS 2D-DIGE Beta fibrin
S100 calcium-binding protein transferrin
cofilin-1
Western
Blotting
LC/MS    
Cleft lip
and palate[42]
31&20 USS WS MALDI-TOF/MS Arpc3 Dermokine  
Gingivitis[43] 10&10 USS WS LC/MS MALDI-TOF/MS Albumin  Hemoglobin
Immunoglobulin
Keratins
 
OSCC[44] 4&4 USS WS   Myosin
Actin
Western
Blotting
Chronic periodontitis[45] 10&10 USS WS 2D-DIGE MALDI-TOF/MS
nLC-MS/MS
Immunoglobulin
Hemoglobin
Albumin
Cystatin
 
Dental caries[46] 16&16 USS WS 2DE
MALDI-TOF/MS
Amylase
IgA
Lactoferrin
 
Sjögren’s syndrome[47] 8&8 USS WS 2DE
ESI-MS/MS
Keratin
Albumin
2 actin isoforms
 
OSCC[48] 35&51 USS WS   IL-1B
IL-8
M2BP
ELISA
Gastric cancer[40] 23&18 USS WS MALDI-TOF/MS 4 proteins (1472.78 Da, 2936.49 Da, 6556.81 Da, and 7081.17 Da)  
Breast cancer[49] 10&10 USS WS 2D-DIGE CA6 Immunoblotting
Rheumatoid arthritis[50] 20&20 USS WS 2DE
MALDI-TOF/MS
2 S100A
Apolipoprotein
a-1
Immunoblotting
Type diabetes[51] 31&31 USS WS LC-ESI-MS S100A9
PRP-1/PRP-3
 
Typediabetes[15,52] 15&5 USS WS iTRAQ
MALDI-TOF/MS
LC-MS/MS
HbA1c
BPI
MMP-9
 
Type II
diabetes[53]
40&10 USS WS LC-MS/MS 5 metabolism proteins ELISA
HNSCC: Head and Neck Squamous Cellcarcinoma; USS: Unstimulated Saliva; WS: Whole Saliva; OSCC: Oral Squamous Cellcarcinoma
Table 1