Figure 5: (A) BMP3 gene expression in Mz-ChA-1 cells 48 hours after transfection with BMP3 plasmid compared to control vector. (B) Forced expression of BMP3 inhibits Mz-ChA-1 cell proliferation as assessed by the BrdU assay and (C) reduces Mz-ChA-1 cell viability as measured by the MTT assay. (D) Transfection with BMP3 plasmid increased Mz-ChA-1 cell apoptosis at 48 hours, as measured by the relative fluorescence units in a caspase 3/7 assay.