Figure 1: Schematic representation of samples and analysis performed on blood collected from normal subjects for ex-vivo analysis and cancer patients for measurement of global gene expression, pSMAD in PBMCs and plasma analytes. Blood collected in CTAD tubes was used to separate PBMCs for ex-vivo expression analysis and pSMAD measurement. Plasma from EDTA tubes was used for analysis of TGF-β1, PF4 and multiple analytes using the Multi Analyte Immunoassay Panel (MIAP). The Tempus blood collection tube was used for whole blood microarray analysis from patient samples.