Antibody Clone Dilution Provider Retrieval method Positive score
ER SP1 Ready to use Ventana HIER pH 6 Allred score ≥ 3
PR 1E2 Ready to use Ventana HIER pH 6 Allred score ≥ 3
HER2 4B5 Ready to use Ventana HIER pH 6 3+
Ki-67 SP6 1/100 BioCare HIER pH 6 >14%
ANXA1 Clone29 1/500 BD HIER pH 9 0-4 low expression
6-12 high expression
FOXA1 Polyclonal 1/500 Abcam HIER pH 6 0-4 low expression
6-12 high expression
VIM              3B4 1/100 Dako HIER pH 6 0-4 low expression
5-12 high expression
ER: Estrogen receptor; PR: Progesterone receptor; HER2: Human epidermal growth factor receptor 2; ANXA1, Annexin A1; FOXA1: Forkhead box protein A1; VIM: Vimentin; HIER: Heat-induced epitope retrieval
Table 2: Antibodies used for immunohistochemistry.