Figure 4: Inhibition of GJIC after a 24 h exposure to BALF is both treatment and strain dependent. (A) Quantitation of SL/DT assay demonstrating significant differences between strain BALF effects in the C10 cells following the SL/DT assay using Lucifer Yellow fluorescent dye. N=3-5 per treatment group, repeated twice. Data were normalized to the medium control and presented as mean + SEM for fraction of control. *, P<0.05 for BHT compared to oil treatment groups; +, p<0.05 for BALB versus BALBLps-d mice. (B) Image of C10 cells treated with BALF from oil-treated BALB mouse; (C) Image of of C10 cells treated with BALF from BHT-treated BALB mouse; (D) Image of C10 cells treated with BALF from oil-treated BALBLps-d mouse; (E) Image of C10 cells treated with BALF from BHT-treated BALBLps-d mouse; (F) Image from C10 cells treated with serum-deprived media alone as control. Magnification is 100X.