Test Samples Exon    18 Exon     19 Exon    20 Exon    21 1068 Normal Normal Altered Normal 629 Normal Deletion Normal Normal 1012 Normal Deletion Normal Normal 1020 Altered Normal Normal Normal 417 Normal Normal Altered Altered 1009 Altered Normal Normal Altered 906 Normal Normal Normal Normal 1035 Normal Normal Normal Normal 1002 Normal Altered Normal Altered 1124 Normal Altered Normal Altered Note: Normal and altered fluorescent signatures refer to fluorescent signatures from the clinical samples that either match or differ from the reference normal fluorescent signatures (see text for details). Deletions listed were identified based on the similarities between the altered and reference normal fluorescent signatures (Supplementary data S2). The only LATE-PCR result that was discordant with the known EGFR mutation status of these samples defined by COLD-PCR/dideoxysequencing is underlined; see text and Supplementary information S2 and S3 for details.

Table 3: Results from blinded analysis of clinical samples using the LATE-PCR EGFR assay.