Figure 2: Isolated RNA from 3 human breast cancer cell lines and one human kidney cell line was reverse transcribed and amplified using EGFL6-specific primers. Bands of the expected nucleotide length were detected in MDAMB- 435 and MDA-MB-468 samples. Additionally, a primer for β-Actin was used as a loading control. Numbered lanes represent the following: 1) Ladder, 2) SK-BR-3, 3) MDA-MB-435, 4) MDA-MB-468, 5) HEK-293.