Figure 3: Total protein from cell lysates and cultured medium from corresponding cells were run on SDS-PAGE gels, transferred to nitrocellulose, and EGFL6 detected by immunoblot. One band was detected at the expected molecular weight for EGFL6 in the cultured supernatant of MDA-MB-435 cells. Numbered lanes represent the following: 1) Ladder, 2) MDA-MB-435 cell lysate, 3) MDA-MB-435 supernatant, 4) MDA-MB-468 cell lysate, 5) MDAMB- 468 supernatant, SK-BR-3 cell lysate, 6) SK-BR-3 supernatant, 7) HEK- 293 cell lysate, 8) HEK-293 supernatant, 9) ladder, 10) loading buffer.