Figure 7: 111In-DOTA-EGFL6 and 111In-DOTA-3-G03 were injected into MDAMB- 435 human breast carcinoma tumor bearing mice. SPECT/CT imaging was acquired 2 h post-injection. Uptake of 111In-DOTA-EGFL6 is consistent with tumor vasculature accumulation. Sequence specificity of the EGFL6 peptide was confirmed by using the 3-G03 peptide, which differs by only 5 amino acids. 3-G03 did not have a tumor uptake pattern similar to EGFL6, but did show similar kidney uptake, which is typical of radiolabeled peptides.