Figure 4: cobas® 4800 BRAF V600 Mutation Test workflow. The assay is based on two major processes: (1) manual isolation and preparation of DNA from the specimen; (2) PCR amplification and detection. Macrodissection is normally required when specimens have <50% tumor but was not performed for this study for any samples. H&E = hematoxylin and eosin; PCR = polymerase chain reaction.