Kit pre-PCR PCR post PCR Detection Analysis
Cobas 15’ (x3) - - 1h 30’ (x2) -
Therascreen 15’ (x4) 3h (x2) 30’ (x5) 20’ (x10) 10’ (x10)
Three and 4 distinct reactions for PCR amplification then 3 and 5 for sequencing were performed using the Cobas® and Therascreen® kits respectively. All steps, as much as possible, are performed in 96-well plates with the exception of detection with Therascreen® that were performed in 24-well plates and the Pyromark® Q24.
Table 2: EGFR analysis on a series of 48 DNA samples