| Kit |
pre-PCR |
PCR |
post PCR |
Detection |
Analysis |
| Cobas |
15’ (x3) |
- |
- |
1h 30’ (x2) |
- |
| Therascreen |
15’ (x4) |
3h (x2) |
30’ (x5) |
20’ (x10) |
10’ (x10) |
| Three and 4 distinct reactions for PCR amplification then 3 and 5 for sequencing were performed using the Cobas® and Therascreen® kits respectively. All steps, as much as possible, are performed in 96-well plates with the exception of detection with Therascreen® that were performed in 24-well plates and the Pyromark® Q24. |
|
