Patient ID Tissues Gene/Mutation Stained Unstained Wild-type CT Mutant CT Wild-type CT Mutant CT 6012 Colorectal MND 25.1   26.3   6165 Colon MND 25.2   24.8   6172 Rectum MND 24.8   24.1   6173 Colon MND 24.8   24.2   1703 Breast PIK3CA, 1047R 26.7 30.3 19.0 23.4 1740 Breast MND 24.8   21.6   1743 Breast PIK3CA, E545K 26.7 30.1 18.8 22.6 1786 Breast MND 25.2   19.9   2282 Breast PIK3CA, E545K 19.8 21.0 18.6 21.7 2286 Breast MND 27.5   18.4   2317 Breast MND 22.9   18.7   2485 Breast MND 18.8   19.0   2643 Breast MND 25.6   18.2   2765 Breast MND 24.1   19.3   3064 Breast MND 27.6   17.6   3528 Breast MND 29.2   18.3   3565 Breast PIK3CA, H1047R 23.3 26.3 18.2 23.3 3582 Breast MND 24.8   18.9   3659 Breast MND 26.1   21.1   3849 Breast MND 21.9   18.5   3920 Breast MND 28.7   18.8   025-A074 Colorectal Kras, G13D 28.2 33.2 24.5 26.2 025-A090 Colorectal Kras,G12A 28.0 28.2 24.9 24.0 025-A027 Colorectal Kras,G12A 28.0 29.9 24.8 26.2 025-A020 Colorectal MND 21.5 32.3 21.0   025-A020 Colorectal MND 24.2 37.6 23.1   025-A072 Colorectal Kras,G13D 22.3 26.0 24.1 25.6 025-A061 Colorectal Nras,Q61R 21.3 22.5 19.6 21.0 025-A121 Colorectal Nras,Q61K 25.2 25.7 21.4 22.8 025-A028 Colorectal BRAF, V600E 16.2 19.0 15.7 19.0 025-A028 Colorectal BRAF,V600E 18.1 21.1 16.8 19.6 CT= cycle threshold; MND = Mutation not detected

Table 1: Correlation between mutation calls made using unstained and IHC-stained sections. Mutation analysis was done using TaqMan (BRAF, NRAS ) and Therascreen® KRAS and PI3K Mutation kits.