Patient ID Tissues Gene/Mutation Stained Section Wild-type CT Mutant CT 025-A090 Colorectal Kras, G12A 1 28.0 28.2     Kras, G12A 2 29.9 30.2 025-A027 Colorectal Kras, G12A 1 28.0 29.9     Kras, G12A 2 29.8 32.1 025-A020 Colorectal MND 1 21.5 32.3     MND 2 23.3 35.9 025-A020 Colorectal MND 1 24.2 37.6     MND 2 24.8 35.1 025-A072 Colorectal Kras, G13D 1 22.3 26.0     Kras, G13D 2 21.9 25.2 025-A061 Colorectal Nras, Q61R 1 21.3 22.5     Nras, Q61R 2 20.0 21.8 025-A121 Colorectal Nras, Q61K 1 25.2 25.7     Nras, Q61K 2 24.7 26.6 025-A028 Colorectal BRAF, V600E 1 16.2 19.0     BRAF, V600E 2 18.4 23.2 025-A028 Colorectal BRAF, V600E 1 18.1 21.1     BRAF, V600E 2 19.4 22.4 CT= cycle threshold; MND = Mutation not de

Table 2: Reproducibility of two consecutive IHC-stained sections. Mutation analysis was done using TaqMan (BRAF, NRAS) and Therascreen® KRAS Mutation kits.