Patient ID Tissues Gene/ Mutation Stained Wild-type CT Mutant CT 6169 Colorectal MND 24.6   4176 Breast No call 30.1 32.7 4417 Breast MND 28.3   4042 Breast PIK3CA, E542K 27.9 32.9 4152 Breast MND 25.7   4126 Breast No call 34.8   4396 Breast No call 33.7   4092 Breast No call 33.1   4367 Breast MND 27.4   4146 Breast No call 32.2   4340 Breast MND 29.2   4332 Breast PIK3CA, H1047R 26.5 29.7 4331 Breast MND 26.9   4098 Breast MND 26.3   4288 Breast MND 27.3   4289 Breast MND 27.4   6203 Colorectal Kras, G12V 26.2 28.2 6014 Colorectal Kras, G12D 25.7 26.6 4081 Colorectal Kras, G12V 29.0 30.7 4002 Colorectal MND 24.3   4091 Colorectal Kras, G12D 25.6 28.5 4088 Colorectal MND 26.3   4087 Colorectal MND 26.0   2302 Breast MND 27.1   3841 Breast MND 29.7   2520 Breast MND 29.3   2362 Breast PIK3CA, E545K/D 27.6 29.65 3803 Breast MND 27.3   2309 Breast No call 31.0   3500 Breast MND 26.5   2503 Breast MND 25.3   3020 Breast MND 26.0   3641 Breast MND 26.6   2044 Breast MND 25.4   4003 Breast PIK3CA, H1047R 29.1 32.51 2363 Breast No call 30.5   3069 Breast No call 30.6   4005 Breast MND 27.2   2342 Breast MND 27.5   3700 Breast MND 25.0   2314 Breast No call 30.4   2529 Breast MND 26.5   3022 Breast MND 29.2   3940 Breast MND 25.6   3703 Breast PIK3CA, E545K/D 24.6 25.7 3701 Breast MND 28.4   4041 Breast PIK3CA, E545K/D 24.1 32.4 3704 Breast No call 31.9   2367 Breast MND 25.6   3560 Breast PIK3CA, E545K/D 29.1 34.3 1961 Breast MND 25.5   2480 Breast MND 25.6   1620 Breast MND 26.6   3605 Breast MND 28.4   2985 Breast MND 22.0   3655 Breast MND 25.7   3653 Breast MND 24.8   3942 Breast MND 23.2   3052 Breast MND 21.4   3658 Breast MND 26.9   2661 Breast MND 22.2   3567 Breast MND 26.5   2767 Breast Nocall 30.4   2160 Breast MND 25.5   3853 Breast MND 25.2   2946 Breast MND 22.8   2145 Breast MND 21.1   2947 Breast MND 24.8   CT= cycle threshold; MND = Mutation not detected

Table 3: Unstained sections not available for these samples, mutation analysis was done using IHC stained sections.