Gene Function Amplification Efficiency (%) R2 SD 95% CI ASF1B CCP 111 0.96 0.80 0.71 , 0.91 ASPM CCP 90 0.97 0.36 0.32 , 0.42 BIRC5 CCP 90 0.99 0.99 0.88 , 1.14 BUB1B CCP 82 0.98 0.30 0.26 , 0.34 C18orf24 CCP 81 0.97 0.71 0.63 , 0.81 CDC2 CCP 108 0.95 0.39 0.35 , 0.45 CDC20 CCP 101 0.95 0.39 0.35 , 0.45 CDCA3 CCP 96 0.96 0.98 0.87 , 1.13 CDCA8 CCP 93 0.99 0.21 0.19 , 0.24 CDKN3 CCP 90 0.98 0.28 0.25 , 0.32 CENPF CCP 81 0.99 0.45 0.40 , 0.51 CENPM CCP 107 0.98 0.50 0.45 , 0.57 CEP55 CCP 93 0.93 0.83 0.73 , 0.95 DLGAP5 CCP 79 0.97 0.49 0.44 , 0.56 DTL CCP 82 0.96 0.50 0.44 , 0.57 FOXM1 CCP 102 0.98 0.32 0.28 , 0.36 KIAA0101 CCP 105 0.96 0.31 0.28 , 0.35 KIF11 CCP 78 0.96 0.58 0.51 , 0.66 KIF20A CCP 94 0.95 0.61 0.54 , 0.69 MCM10 CCP 111 0.96 0.64 0.57 , 0.73 NUSAP1 CCP 105 0.96 0.43 0.38 , 0.49 ORC6L CCP 85 0.97 0.41 0.37 , 0.47 PBK CCP 89 0.97 0.59 0.53 , 0.68 PLK1 CCP 83 0.98 0.33 0.29 , 0.38 PRC1 CCP 91 0.97 0.26 0.23 , 0.30 PTTG1 CCP 98 0.99 0.43 0.38 , 0.49 RAD51 CCP 105 0.94 0.57 0.50 , 0.65 RAD54L CCP 105 0.96 0.41 0.37 , 0.47 RRM2 CCP 90 0.98 0.32 0.29 , 0.37 TK1 CCP 121 0.96 0.28 0.25 , 0.32 TOP2A CCP 84 0.97 0.65 0.58 , 0.74 Median   93 0.97 0.43   CLTC HK 107 0.95 0.26 0.23 , 0.29 MMADHC HK 107 0.95 0.32 0.29 , 0.37 MRFAP1 HK 117 0.95 0.24 0.21 , 0.27 PPP2CA HK 107 0.97 0.29 0.26 , 0.33 PSMA1 HK 91 0.98 0.23 0.20 , 0.26 PSMC1 HK 85 0.98 0.32 0.28 , 0.36 RPL13A HK 102 0.97 0.27 0.24 , 0.31 RPL37 HK 82 0.99 0.27 0.24 , 0.31 RPL38 HK 92 0.97 0.25 0.22 , 0.28 RPL4 HK 86 0.99 0.24 0.21 , 0.27 RPL8 HK 100 0.96 0.27 0.24 , 0.31 RPS29 HK 117 0.96 0.22 0.20 , 0.25 SLC25A3 HK 102 0.97 0.19 0.17 , 0.22 TXNL1 HK 83 0.97 0.27 0.24 , 0.31 UBA52 HK 88 0.98 0.21 0.19 , 0.24 Median   100 0.97 0.26

SD, standard deviation. CI, confidence interval. CCP, cell cycle progression. HK, housekeeper. The listed R2 value is for the fit when determining the amplification efficiency for each amplicon.

Table 1: List of genes, their function within the gene signature, as well as the amplification efficiencies and SD of the CT and ΔCT for individual housekeeper and target genes.