KRAS Mutation Sample ID Mutation
(%)
Lowest DNA Input with Correct Call a (ng/PCR)
G12C, GGT>TGT FFPET #1, DNA blend 6.2 12.5
FFPET #2, DNA blend 6.0 3.1
FFPET #3, DNA blend 5.6 3.1
Cell line (NCI-H23),
DNA blend #1
12.1 0.8
Cell line (NCI-H23),
DNA blend #2
6.1 3.1
Cell line (NCI-H23),
DNA blend #3
2.4 50.0
G13C, GGC>TGC FFPET #4, DNA blend 6.2 3.1
FFPET #5, DNA blend 4.4 3.1
FFPET #6, DNA blend 4.4 3.1
Cell line (NCI-H1734),
DNA blend #4
7.8 0.8
Cell line (NCI-H1734),
DNA blend #5
4.9 3.1
Cell line (NCI-H1734),
DNA blend #6
2.8 3.1
Q61H, CAA>CAT FFPET #7, DNA blend 4.5 3.1
Cell line (NCI-H460),
DNA blend #7
10.2 0.8
Cell line (NCI-H460),
DNA blend #8
6.1 0.8
Cell line (NCI-H460),
DNA blend #9
2.7 12.5
Q61H, CAA>CAC FFPET #8, DNA blend 6.4 3.1
Q61L, CAA>CTA FFPET #9, DNA blend 6.5 3.1
aLowest amount of input DNA (ng/PCR) tested that yielded a correct mutation call rate of ≥ 95% (upper one-sided 95% CI). Each test used 72 replicates for cell line DNA blends and 24 replicates for FFPET DNA blends.
Table 1: Summary of LOD results for the predominant KRAS mutations in codons 12, 13 and 61.