| KRAS Mutation |
Sample ID |
Mutation
(%) |
Lowest DNA Input with Correct Call a (ng/PCR) |
| G12C, GGT>TGT |
FFPET #1, DNA blend |
6.2 |
12.5 |
| FFPET #2, DNA blend |
6.0 |
3.1 |
| FFPET #3, DNA blend |
5.6 |
3.1 |
Cell line (NCI-H23),
DNA blend #1 |
12.1 |
0.8 |
Cell line (NCI-H23),
DNA blend #2 |
6.1 |
3.1 |
Cell line (NCI-H23),
DNA blend #3 |
2.4 |
50.0 |
| G13C, GGC>TGC |
FFPET #4, DNA blend |
6.2 |
3.1 |
| FFPET #5, DNA blend |
4.4 |
3.1 |
| FFPET #6, DNA blend |
4.4 |
3.1 |
Cell line (NCI-H1734),
DNA blend #4 |
7.8 |
0.8 |
Cell line (NCI-H1734),
DNA blend #5 |
4.9 |
3.1 |
Cell line (NCI-H1734),
DNA blend #6 |
2.8 |
3.1 |
| Q61H, CAA>CAT |
FFPET #7, DNA blend |
4.5 |
3.1 |
Cell line (NCI-H460),
DNA blend #7 |
10.2 |
0.8 |
Cell line (NCI-H460),
DNA blend #8 |
6.1 |
0.8 |
Cell line (NCI-H460),
DNA blend #9 |
2.7 |
12.5 |
| Q61H, CAA>CAC |
FFPET #8, DNA blend |
6.4 |
3.1 |
| Q61L, CAA>CTA |
FFPET #9, DNA blend |
6.5 |
3.1 |
| aLowest amount of input DNA (ng/PCR) tested that yielded a correct mutation call rate of ≥ 95% (upper one-sided 95% CI). Each test used 72 replicates for cell line DNA blends and 24 replicates for FFPET DNA blends. |
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